Aktivitas antioksidan ekstrak air daun kelor (Moringa oleifera) dengan metode DPPH serta analisis kualitatif kandungan metabolit sekunder
DOI:
10.29303/sjp.v3i2.182Downloads
Abstract
Sunlight contains UV radiation which will have an impact on the skin if exposed continuously and cause premature aging of the skin. The use of cosmetic products that contain antioxidant compounds is needed to avoid premature aging due to exposure to UV rays. Antioxidants used in cosmetics generally come from synthetic compounds, namely BHT (Butyl hydroxytoluene) whose use will have a negative impact on the skin if used in the long term, so in this study, Moringa oleifera leaf was used as a natural antioxidant. The purpose of this study is to determine the antioxidant activity of Moringa leaf water extract by calculating IC50 and using a comparison standard of vitamin C. The method used in this study is the stratified maceration method with measurement of antioxidant activity using the DPPH method. The results showed that the aqueous extract of Moringa leaves contains secondary metabolites of flavonoids, tannins, saponins, and alkaloids that play a role in antioxidant activity. The test results of Moringa leaf water extract showed moderate antioxidant activity with an IC50 value of 87.54 ppm, while the comparison control vitamin C obtained an IC50 of 483.53 ppm. This is because vitamin C has been oxidized so that the ability to donate protons to DPPH free radicals is also reduced.
Keywords:
Moringa leaf water extract, antioxidant, DPPH, ascorbic acid, graded macerationReferences
Alim, N., Jummah, N., Pratama, A. S., & Nurdiyanti, N. (2021). Skirining fitokimia ekstrak etanol kulit buah sirsak (Annona muricata Linn) dan uji aktivitas antioksidan dengan metode DPPH. Sasambo Journal of Pharmacy, 2(2), 60–64. https://doi.org/10.29303/sjp.v2i2.40
Amin, M. A., Anwar, & Haeruddin. (2018). Pengaruh Bauran Pemasaran Jasa Terhadap Minat Kembali Pasien Non Asuransi di Poli Rawat Jalan Rumah Sakit Umum Daerah Daya Kota Makassar. Jurnal Mitrasehat, 8(2), 479–488.
Aminah, Maryam, S., Baits, M., & Kalsum, U. (2016). Perbandingan aktivitas antioksidan ekstrak etanol daun sirsak (Annona muricata L.) berdasarkan tempat tumbuh dengan metode peredaman DPPH. Jurnal Fitofarmaka Indonesia, 3(1), 146–150.
Bhattacharya, A. T. P. S. P. K. S. (2018). Review of the Phytochemical and Pharmacological Characteristics of Moringa oleifera. J Pharm Bioallied Sci, 10(4), 181–191.
Cresna, Napitupulu, M., & Ratman. (2014). Analysis of Vitamin C in The Fruit of Papaya, Soursop, Sugar Apple and Langsat That Grown in Donggala. Jurnal Akademika Kimia, 3(3), 58–65.
Fitriana, W. D., Fatmawati, S., & Ersam, T. (2015). Uji Aktivitas Antioksidan terhadap DPPH dan ABTS dari Fraksi-fraksi. SNIP Bandung, 2015(Snips), 658.
Haeria, Ningsi, S., & Israyani. (2014). Penentuan Potensi Tabir Surya Ekstrak Klika Anak Dara (Croton oblongus Burm F.). Jurnal Farmasi Fakultas Ilmu Kesehatan Universitas Islam Negeri Alauddin Makassar, Vol. 2(1), 1–5.
Leone, A., Spada, A., Battezzati, A., Schiraldi, A., Aristil, J., & Bertoli, S. (2015). Cultivation, genetic, ethnopharmacology, phytochemistry and pharmacology of Moringa oleifera leaves: An overview. International Journal of Molecular Sciences, 16(6), 12791–12835. https://doi.org/10.3390/ijms160612791
Maesaroh, K., Kurnia, D., & Al Anshori, J. (2018). Perbandingan Metode Uji Aktivitas Antioksidan DPPH, FRAP dan FIC Terhadap Asam Askorbat, Asam Galat dan Kuersetin. Chimica et Natura Acta, 6(2), 93. https://doi.org/10.24198/cna.v6.n2.19049
Malangngi, L., Sangi, M., & Paendong, J. (2012). Penentuan Kandungan Tanin dan Uji Aktivitas Antioksidan Ekstrak Biji Buah Alpukat (Persea americana Mill.). Jurnal MIPA, 1(1), 5. https://doi.org/10.35799/jm.1.1.2012.423
Nurjana, Abdullah, A., & Apriandi, A. (2011). Aktivitas Antioksidan Dan Komponen Bioaktif Keong Ipong-Ipong (Fasciolaria salmo). Jurnal Pengolahan Hasil Perikanan Indonesia, 15(1), 22–29.
Paikra, B. K., Dhongade, H. K. J., & Gidwani, B. (2017). Phytochemistry and pharmacology of Moringa oleifera Lam. Journal of Pharmacopuncture, 20(3), 194–200. https://doi.org/10.3831/KPI.2017.20.022
Park, M. O., Park, C. Il, Jin, S. J., Park, M. R., Choi, I. Y., Park, C. H., & Adnan, M. (2022). Comparison in Content of Total Polyphenol, Flavonoid, and Antioxidant Capacity from Different Organs and Extruded Condition of Moringa oleifera Lam. Processes, 10(5). https://doi.org/10.3390/pr10050819
Prasanth, D., Sreedhara, C. S., Deepak, M., Sangli, V. K., & ... (2011). Comparitive study on estimation of polyphenols in different extracts of Moringa oleifera leaves and fruits with respect to tannic acid. J Pharm Res, August 2014, 1–3. https://www.researchgate.net/profile/Prasanth_Dintakurthi/publication/265014843_Comparitive_study_on_estimation_of_polyphenols_in_different_extracts_of_Moringa_oleifera_leaves_and_fruits_with_respect_to_tannic_acid/links/53fc05410cf2364ccc046463/Compariti
Rahmayani, U., Pringgenies, D., & Djunaedi, A. (2013). Uji Aktivitas Antioksidan Ekstrak Kasar Keong Bakau (Telescopium telescopium) dengan Pelarut yang Berbeda terhadap Metode DPPH (Diphenyl Picril Hidrazil). Journal Of Marine Research, 2(4), 36–45. http://ejournal-s1.undip.ac.id/index.php/jmr
Riskianto, Kamal, S. E., & Aris, M. (2021). Aktivitas antioksidan ekstrak etanol 70% daun kelor (Moringa oleifera Lam.) terhadap DPPH. Jurnal Pro-Life, 8(2), 168–177.
Salim, R., & Eliyarti, E. (2019). Aktivitas Antioksidan Infusa Daun Kelor (Moringa oleifera Lam.) Terhadap Warna Daun. Jurnal Katalisator, 4(2), 91. https://doi.org/10.22216/jk.v4i2.4210
Suryanto, E., & Wehantouw, F. (2009). Aktivitas Penangkap Radikal Bebas dari Ekstrak Fenolik Daun Sukun (Artocarpus altilis F.). Chemistry Progress, 2(1), 1–7.
Verdiana, M., Widarta, I. W. R., & Permana, I. D. G. M. (2018). Pengaruh jenis pelarut pada ekstraksi menggunakan gelombang ultrasonik terhadap aktivitas antioksidan ekstrak kulit buah lemon (Citrus limon (Linn.) Burm F.). Jurnal Ilmu Dan Teknologi Pangan (ITEPA), 7(4), 213. https://doi.org/10.24843/itepa.2018.v07.i04.p08
Werdhasari, A. (2014). Peran Antioksidan Bagi Kesehatan. Jurnal Biomedik Medisiana Indonesia, 3(2), 59–68.
Widyowati, H., Ulfah, M., & Sumantri. (2014). Uji Aktivitas Antioksidan Ekstrak Etanolik Herba Alfalfa (Medicago sativa L.) dengan Metode DPPH (1,1-Diphenyl-2 Picrylhidrazyl). Jurnal Ilmu Farmasi Dan Farmasi Klinik, 11(1), 25–33.
Yuliani, N. N., & Dienina, D. P. (2015). Uji aktivitas antioksidan infusa daun kelor (Infusa Moringa). Jurnal Info Kesehatan, 14(2), 1060–1082.
Zengin, G., Aktumsek, A., Guler, G. O., Cakmak, Y. S., & Yildiztugay, E. (2011). Antioxidant properties of methanolic extract and fatty acid composition of Centaurea urvillei DC. subsp. hayekiana Wagenitz. Records of Natural Products, 5(2), 123–132.
License
Copyright (c) 2022 The Author(s)
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with Sasambo Journal of Pharmacy (SJP), agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Lisensi Creative Commons Atribusi 4.0 Internasional. This license allows authors to use all articles, data sets, graphics and appendices in data mining applications, search engines, web sites, blogs, and other platforms by providing an appropriate reference. The journal allows the author(s) to hold the copyright without restrictions and will retain publishing rights without restrictions.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in Sasambo Journal of Pharmacy
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
