Vol. 4 No. 1 (2023): April
Open Access
Peer Reviewed

Penetapan kadar vitamin C pada daun kelor (Moringa oleifera L.) dan bayam hijau (Amaranthus gangeticus L.) dengan metode spektrofotometri UV-Vis

Authors

Siti Maryam Indriyati , Yayuk Andayani , Anggit Listyacahyani Sunarwidhi

DOI:

10.29303/sjp.v4i1.190

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Received: Nov 07, 2022
Accepted: Feb 16, 2023
Published: Apr 30, 2023

Abstract

Vitamin C is a compound that cannot be formed by the body itself. Vitamin C is needed to regulate metabolic processes and normal body functions. Lack of vitamin C can cause a decrease in the immune system. During a pandemic, vitamin C is needed to improve the immune system to prevent and fight disease infections. The pandemic has caused an increase in the need and price of vitamin C supplements in the market. Fulfilling the body’s vitamin C needs can use alternatives such as Moringa leaves and green spinach. This study aims to determine the levels of vitamin C in Moringa leaves and green spinach with variations in temperature and concentration. Analysis of vitamin C was carried out using UV-Vis spectrophotometry at a wavelength of 516 nm. The results of the determination of vitamin C levels in Moringa leaves and green spinach obtained the highest levels of 24,9948% and 19,0205%, and the lowest levels of 2,2456% and 1,8710%. The results of the ANOVA test showed that there was a significant difference in the levels of vitamin C obtained at the given temperature and concentration variations.

Keywords:

Vitamin C, Moringa leaves, Green spinach, UV-Vis spectrophotometry

References

Arel, A., Martinus, B. A., & Ningrum, S. A. (2017). Penetapan Kadar Vitamin C pada Buah Naga Merah (Hylocereus costaricensis (FAC Weber) Britton & Rose) dengan Metode Spektrofotometri UV-Visibel. Scientia: Jurnal Farmasi Dan Kesehatan, 7(1), 1–5.

Badan Pengawas Obat dan Makanan. (2020). Pedoman Penggunaan Herbal dan Suplemen Kesehatan dalam Menghadapi COVID-19 di Indonesia. BPOM RI. https://www.jamudigital.com/download/BukuSakuObatHerbalSaatCovid19.pdf

Cahyati, R. D., Natsir, H., & Wahab, A. W. (2014). Analisis Kadar Asam Askorbat dalam Ekstrak Daun Kelor (Moringa Oleifera Lam) dari Daerah Pesisir dan Pegunungan Serta Potensinya Sebagai Antioksidan. Makassar: Universitas Hasanudin.

Dong, J., Ma, X., Wei, Q., Peng, S., & Zhang, S. (2011). Effects of Growing Location on the Contents of Secondary Metabolites in the Leaves of Four Selected Superior Clones of Eucommia ulmoides. Industrial Crops and Products, 34(3), 1607–1614. https://doi.org/10.1016/j.indcrop.2011.06.007

Fatimah, S., Hadju, V., Bahar, B., & Abdullah, Z. (2011). Pola Konsumsi dan Kadar Hemoglobin pada Ibu Hamil di Kabupaten Maros, Sulawesi Selatan. Makara Kesehatan, 15(1), 31–36. https://dx.doi.org/10.7454/msk.v15i1.795

Food and Drug Administration. (2020). ORA Laboratory Manual Volume II : Methods, Method Verification and Validation. US Department of Health and Human Service.

González, A. G., & Herrador, M. Ã. (2007). A Practical Guide to Analytical Method Validation, Including Measurement Uncertainty and Accuracy Profiles. TrAC Trends in Analytical Chemistry, 26(3), 227–238. https://doi.org/10.1016/j.trac.2007.01.009

Handayani, R., Dhasefa, D. A., Sari, M. W., Sukmawan, M. S., & Rofiah, N. (2020). Pembuatan Smoothies Mangga Sebagai Imun Booster Bagi Warga Kota Kulon Kabupaten Garut. Aptekmas: Jurnal Pengabdian Kepada Masyarakat, 3(4). https://doi.org/10.36257/apts.v3i4.2962

Harmita, H. (2012). Petunjuk Pelaksanaan Validasi Metode dan Cara Perhitungannya. Majalah Ilmu Kefarmasian, 1(3), 117–135. https://doi.org/10.7454/psr.v1i3.3375

Hidayah, S. N., Izah, N., & Andari, I. D. (2020). Peningkatan Imunitas dengan Konsumsi Vitamin C dan Gizi Seimbang Bagi Ibu Hamil Untuk Cegah Corona Di Kota Tegal. Jurnal ABDINUS: Jurnal Pengabdian Nusantara, 4(1), 170–174. https://doi.org/10.29407/ja.v4i1.14641

I Nengah, B. S., Ahmad, F. A., Chrysella, R., Farah, K., Fitria, F., Happy, N. E. S., Hieronimus, A. N. U., Safiinatunnajah, N.,

Wahyu, A. D., Yunita, A., & Rahem, A. (2020). Hubungan Usia dengan Pengetahuan dan Perilaku Penggunaan Suplemen pada Mahasiswa Institut Teknologi Sepuluh Nopember. Jurnal Farmasi Komunitas, 7(1), 1–7.

Kamiensky, M., & Keogh, J. (2015). Farmakologi Demystified. Rapha Publishing.

Karamoy, L. T. (2009). Hubungan Iklim Dengan Pertumbuhan Kedelai (Glycine max L Merrill). Soil Environment, 7(1), 65–68.

Kementerian Perindustrian R. I. (2019). Analisis Perkembangan Industri. Kementrian Kesehatan RI.

Mahmood, K. T., Mugal, T., & Haq, I. U. (2010). Moringa oleifera: A Natural Gift-A Review. Journal of Pharmaceutical Sciences and Research, 2(11), 775. https://www.researchgate.net/publication/289088517

Manuhara, A. (2017). Penetapan Kadar Vitamin C Pada Daun Bayam Hijau (Amaranthus Tricolor L) Segar, Rebus Dan Goreng Secara Spektrofotometri Uv-Vis. Universitas Setia Budi

Permenkes, R. I. (2013). Peraturan Menteri Kesehatan Republik Indonesia No 75 Tahun 2013 tentang Angka Kecukupan Gizi yang Dianjurkan Bagi Bangsa Indonesia. Kemenkes RI. https://peraturan.bpk.go.id/Home/Details/139226/permenkes-no-75-tahun-2013

Poedjiadi, A., & Supriyanti, F. M. T. (1994). Dasar-dasar Biokimia. Universitas Indonesia Press.

Ronis, M. J. J., Pedersen, K. B., & Watt, J. (2018). Adverse Effects of Nutraceuticals and Dietary Supplements. Annual

Review of Pharmacology and Toxicology, 58, 583–601. https://doi.org/10.1146/annurev-pharmtox-010617-052844

Rukmana, I. H. R. (1994). Bayam, Bertanam & Pengolahan Pascapanen. Kanisius.

Tahir, M., Hikmah, N., & Rahmawati, R. (2016). Analisis Kandungan Vitamin C dan β-Karoten dalam Daun Kelor (Moringa Oleifra Lam.) dengan Metode Spektrofotometri Uv–vis. Jurnal Fitofarmaka Indonesia, 3(1), 135–140. https://doi.org/10.33096/jffi.v3i1.173

Wardani, L. A. (2012). Validasi Metode Analisis dan Penentuan Kadar Vitamin C Pada Minuman Buah Kemasan dengan Spektrofotometri Uv-Visible. Universitas Indonesia.

Author Biographies

Siti Maryam Indriyati, Universitas Mataram

Yayuk Andayani, Universitas Mataram

Anggit Listyacahyani Sunarwidhi, Universitas Mataram

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How to Cite

Indriyati, S. M., Andayani, Y., & Sunarwidhi, A. L. (2023). Penetapan kadar vitamin C pada daun kelor (Moringa oleifera L.) dan bayam hijau (Amaranthus gangeticus L.) dengan metode spektrofotometri UV-Vis. Sasambo Journal of Pharmacy, 4(1), 1–7. https://doi.org/10.29303/sjp.v4i1.190