Influence of extraction method on total phenolic content and antioxidant activity of Sappan Wood (Caesalpinia sappan L.) extract

Tubagus Akmal; Yenni Puspita  Tanjung; Andi Ika Julianti; Aulia Gustiani  Lestari; Aljan Aljan

doi:10.29303/sjp.v5i2.364

Influence of extraction method on total phenolic content and antioxidant activity of Sappan Wood (Caesalpinia sappan L.) extract

Authors

Tubagus Akmal , Yenni Puspita Tanjung , Andi Ika Julianti , Aulia Gustiani Lestari , Aljan Aljan

DOI:

10.29303/sjp.v5i2.364

Published:

2024-09-30

Issue:

Vol. 5 No. 2 (2024): September

Keywords:

Antioxidant, Caesalpinia sappan, Extraction method, Total phenolic content, Ultrasound assisted extraction

Articles

Downloads

PDF

How to Cite

Akmal, T., Tanjung, Y. P. ., Julianti, A. I., Lestari, A. G. ., & Aljan, A. (2024). Influence of extraction method on total phenolic content and antioxidant activity of Sappan Wood (Caesalpinia sappan L.) extract . Sasambo Journal of Pharmacy, 5(2), 55–62. https://doi.org/10.29303/sjp.v5i2.364

Download Citation

Abstract

Sappan wood is a botanical species that contains a high concentration of phenolic compounds, which contribute to several pharmacological properties, including antidiabetic, antibacterial, antioxidant, and wound-healing activities. In order to achieve phenolic compounds of high quality and quantity, it is crucial to select an appropriate extraction procedure. Plant extraction is an important step for chemical isolation, chemical analysis, and evaluating the biological and pharmacological activities of plant compounds. Therefore, determining the most favorable extraction conditions is a critical undertaking in order to maximize both the quantity of active plant compounds and the extraction yield. This research aimed to compare extraction methods based on total phenolic content (TPC) and antioxidant activity (IC50). The extraction techniques employed include maceration, stirring-assisted extraction (SAE), ultrasound-assisted extraction (UAE), and microwave-assisted extraction (MAE). The results show TPC values for maceration, SAE, UAE, and MAE, which are 132.85±1.44, 150.99±1.69, 206.70±6.56, and 115.70±1.44 mg GAE/g DE, respectively. The DPPH antioxidant activity (IC50) values are 32.33±2.64, 25.01±0.34, 20.68±0.29, and 29.18±0.70 µg/mL, respectively. The research findings indicate that various extraction procedures can impact the extraction yield, total phenolic content (TPC), and antioxidant activity (IC50) of sappan wood, and UAE is the best extraction method.

References

Akmal, T., Julianti, A. I., & Syamsudin, S. S. (2023). Polyherbal Formulation Optimization From Clitoria Ternatea, Rosmarinus Officinalis and Aquilaria Malaccensis Using Simplex Lattice Design. International Journal of Applied Pharmaceutics, 15(Special Issue 2), 79–84. https://doi.org/10.22159/ijap.2023.v15s2.15

Akmal, T., Julianti, A. I., Tanjung, Y. P., Mutiara, P., & Febriyanti, S. (2024). Effect of extraction method on total phenolic content and antioxidant activity of Terminalia catappa ( L .) leaves The impact of technological advancements on human beings is not always. PHARMASIPHA : Pharmaceutical Journal of Islamic Pharmacy, 8(2), 1–11.

Arsiningtyas, I. S. (2021). Antioxidant Profile of Heartwood and Sapwood of Caesalpinia sappan L. Tree’s Part Grown in Imogiri Nature Preserve, Yogyakarta. IOP Conference Series: Earth and Environmental Science, 810(1). https://doi.org/10.1088/1755-1315/810/1/012040

Bachtler, S., & Bart, H. J. (2021). Increase the yield of bioactive compounds from elder bark and annatto seeds using ultrasound and microwave assisted extraction technologies. Food and Bioproducts Processing, 125, 1–13. https://doi.org/10.1016/j.fbp.2020.10.009

Bagade, S. B., & Patil, M. (2021). Recent Advances in Microwave Assisted Extraction of Bioactive Compounds from Complex Herbal Samples: A Review. Critical Reviews in Analytical Chemistry, 51(2), 138–149. https://doi.org/10.1080/10408347.2019.1686966

Chemat, F., Rombaut, N., Sicaire, A. G., Meullemiestre, A., Fabiano-Tixier, A. S., & Abert-Vian, M. (2017). Ultrasound assisted extraction of food and natural products. Mechanisms, techniques, combinations, protocols and applications. A review. Ultrasonics Sonochemistry, 34, 540–560. https://doi.org/10.1016/J.ULTSONCH.2016.06.035

Chen, J., Yang, J., Ma, L., Li, J., Shahzad, nasir, & Kyung Kim, chan. (2020). Structure-antioxidant activity relationship of methoxy, phenolic hydroxyl, and carboxylic acid groups of phenolic acids. Scientific RepoRtS |, 10, 2611. https://doi.org/10.1038/s41598-020-59451-z

Da Porto, C., Porretto, E., & Decorti, D. (2013). Comparison of ultrasound-assisted extraction with conventional extraction methods of oil and polyphenols from grape (Vitis vinifera L.) seeds. Ultrasonics Sonochemistry, 20(4), 1076–1080. https://doi.org/10.1016/j.ultsonch.2012.12.002

de Sousa, C. B. do C., dos Anjos, G. L., Nóbrega, R. S. A., da S. Magaton, A., de Miranda, F. M., & de S. Dias, F. (2020). Greener ultrasound-assisted extraction of bioactive phenolic compounds in Croton heliotropiifolius Kunth leaves. Microchemical Journal, 159(September). https://doi.org/10.1016/j.microc.2020.105525

Esquivel-Hernández, D. A., Ibarra-Garza, I. P., Rodríguez-Rodríguez, J., Cuéllar-Bermúdez, S. P., Rostro-Alanis, M. de J., Alemán-Nava, G. S., García-Pérez, J. S., & Parra-Saldívar, R. (2017). Green extraction technologies for high-value metabolites from algae: a review. Biofuels Bioproducts Biorefining. https://doi.org/10.1002/BBB

Firdawati, N., Kuntana, Y. P., Ghozali, M., Utama, G. L., Panigoro, R., & Safitri, R. (2022). Antioxidant Activity of Sappan Wood Extract (Caesalpinia sappan L.) As An Adjuvant and Substitution in Blood Serum of Rats (Rattus norvegicus) Iron Overload Model. IOP Conference Series: Earth and Environmental Science, 1211(1). https://doi.org/10.1088/1755-1315/1211/1/012006

Gomez, L., Tiwari, B., & Garcia-Vaquero, M. (2020). Emerging extraction techniques: Microwave-assisted extraction. In Sustainable Seaweed Technologies: Cultivation, Biorefinery, and Applications (pp. 207–224). Elsevier Inc. https://doi.org/10.1016/B978-0-12-817943-7.00008-1

Hassanpour, S. H., & Doroudi, A. (2023). Review of the antioxidant potential of flavonoids as a subgroup of polyphenols and partial substitute for synthetic antioxidants. Avicenna Journal of Phytomedicine, 13(4), 354–376. https://doi.org/10.22038/AJP.2023.21774

Irfan, S., Ranjha, M. M. A. N., Nadeem, M., Safdar, M. N., Jabbar, S., Mahmood, S., Murtaza, M. A., Ameer, K., & Ibrahim,

S. A. (2022). Antioxidant Activity and Phenolic Content of Sonication- and Maceration-Assisted Ethanol and Acetone Extracts of Cymbopogon citratus Leaves. Separations, 9(9), 244. https://doi.org/10.3390/separations9090244

Kementrian Kesehatan Republik Indonesia. (2017). Farmakope Herbal Indonesia. In Kementrian Kesehatan Republik Indonesia. https://doi.org/10.2307/jj.2430657.12

Lamadjido, S. R., Umrah, U., & Jamaluddin, J. (2019). Formulasi dan Analisis Nilai Gizi Bakso Kotak dari Jamur Tiram Putih (Pleurotus Ostreatus). Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal), 5(2), 166–174. https://doi.org/10.22487/j24428744.2019.v5.i2.13149

Lucena, R. (2012). Extraction and stirring integrated techniques: Examples and recent advances. Analytical and Bioanalytical Chemistry, 403(8), 2213–2223. https://doi.org/10.1007/s00216-012-5826-9

Mnayer, D., Fabiano-Tixier, A.-S., Petitcolas, E., Ruiz, K., Hamieh, T., & Chemat, F. (2017). Extraction of green absolute from thyme using ultrasound and sunflower oil. Resource-Efficient Technologies, 3(1), 12–21. https://doi.org/10.1016/j.reffit.2017.01.007

Mohamad, M., Ali, M. W., Ripin, A., & Ahmad, A. (2013). Effect of extraction process parameters on the yield of bioactive compounds from the roots of eurycoma longifolia. Jurnal Teknologi (Sciences and Engineering), 60(February), 51–57. https://doi.org/10.11113/jt.v60.1441

Mokrani, A., & Madani, K. (2016). Effect of solvent, time and temperature on the extraction of phenolic compounds and antioxidant capacity of peach (Prunus persica L.) fruit. Separation and Purification Technology, 162, 68–76. https://doi.org/10.1016/j.seppur.2016.01.043

Nguyen, V. B., Vu, B. D., Pham, G. K., Le, B. Q., Nguyen, V. C., Men, C. Van, & Nguyen, V. T. (2020). Phenolic Compounds from Caesalpinia sappan. Pharmacognosy Journal, 12(2), 410–414. https://doi.org/10.5530/pj.2020.12.63

Nurul Mahmudati, Hawin Nurdiana, & Poncojari Wahyono. (2022). Antioxidant activity and phenolic content of ginger (Zingiber officinale Roscoe) combination with cinnamon (Cinnamomum burmanii) and Sappan wood (Caesalpinia sappan) as an anti-diabetic. GSC Biological and Pharmaceutical Sciences, 20(3), 001–005. https://doi.org/10.30574/gscbps.2022.20.3.0336

Piang-Siong, W., de Caro, P., Marvilliers, A., Chasseray, X., Payet, B., Shum Cheong Sing, A., & Illien, B. (2017). Contribution of trans-aconitic acid to DPPH scavenging ability in different media. Food Chemistry, 214, 447–452. https://doi.org/10.1016/J.FOODCHEM.2016.07.083

Purba, B. A. V., Pujiarti, R.-, Masendra, M., & Lukmandaru, G. (2023). Total Phenolic, Flavonoid, Tannin Content and DPPH Scavenging Activity of Caesalpinia sappan Linn. Bark. Wood Research Journal, 13(2), 63–68. https://doi.org/10.51850/wrj.2022.13.2.63-68

Putri, D. K., & Sabila, F. I. (2021). Antioxidant activity from the combination ethanol extract secang wood (Caesalpinia sappan L.) and red ginger rhizome (Zingiber officinale Roxb.). International Joint Conference on Science and Engineering 2021 (IJCSE 2021), 209(Ijcse), 143–147. https://www.atlantis-press.com/proceedings/ijcse-21/125966463

Qiu, L., Zhang, M., Chitrakar, B., & Bhandari, B. (2020). Application of power ultrasound in freezing and thawing Processes: Effect on process efficiency and product quality. Ultrasonics Sonochemistry, 68, 105230. https://doi.org/10.1016/J.ULTSONCH.2020.105230

Rifkia, V., & Prabowo, I. (2020). Pengaruh Variasi Suhu dan Waktu terhadap Rendemen dan Kadar Total Flavonoid pada Ekstraksi Daun Moringa oleifera Lam. dengan Metode Ultrasonik The Effect of Temperature and Time of Extraction on the Yield and Total Flavonoid Content of Moringa oleifera La. Pharmaceutical Journal of Indonesia, 17(02), 387–395.

Setyawan, K. N. Y., & Kartini, K. (2023). Optimization of stirring-assisted extraction of anthocyanins from purple roselle (Hibiscus sabdariffa L.) calyces as pharmaceutical and food colorants. Journal of Applied Biology and Biotechnology, 11(5), 91–97. https://doi.org/10.7324/JABB.2023.11510-1

Suksaeree, J., Navabhatra, A., Wunnakup, T., & Monton, C. (2022). Synergistic Antioxidant Activity and Optimal Microwave-Assisted Extraction Condition of Caesalpinia sappan L., Hibiscus sabdariffa L., and Clitoria ternatea L. Combinations. Trends in Sciences, 19(24). https://doi.org/10.48048/tis.2022.3265

Sun, C., Wu, Z., Wang, Z., & Zhang, H. (2015). Effect of ethanol/water solvents on phenolic profiles and antioxidant properties of Beijing propolis extracts. Evidence-Based Complementary and Alternative Medicine, 2015. https://doi.org/10.1155/2015/595393

Suwan, T., Wanachantararak, P., Khongkhunthian, S., & Okonogi, S. (2018). Antioxidant activity and potential of

Caesalpinia sappan aqueous extract on synthesis of silver nanoparticles. Drug Discoveries & Therapeutics, 12(5), 259–266. https://doi.org/10.5582/ddt.2018.01059

Tiwari, B. K. (2015). Ultrasound: A clean, green extraction technology. TrAC Trends in Analytical Chemistry, 71, 100–109. https://doi.org/10.1016/J.TRAC.2015.04.013

Vardhani, A. K. (2019). Caesalpinia sappan L. Proceedings of International Conference on Applied Science and Health, 4, 290–299.

Vij, T., Anil, P. P., Shams, R., Dash, K. K., Kalsi, R., Pandey, V. K., Harsányi, E., Kovács, B., & Shaikh, A. M. (2023). A Comprehensive Review on Bioactive Compounds Found in Caesalpinia sappan. Molecules, 28(17). https://doi.org/10.3390/molecules28176247

Vivekananda Mandal, Yogesh Mohan, S. H. (2007). Microwave Assisted Extraction - An innovative and promising extraction tool for medicinal PHCOG REV .: Review article extraction tool for medicinal plant research. Pharmacognosy Magazine, 1(1), 7–18.

Wen, C., Zhang, J., Zhang, H., Dzah, C. S., Zandile, M., Duan, Y., Ma, H., & Luo, X. (2018). Advances in ultrasound assisted extraction of bioactive compounds from cash crops – A review. Ultrasonics Sonochemistry, 48, 538–549. https://doi.org/10.1016/J.ULTSONCH.2018.07.018

Wijaya, H., Novitasari, & Jubaidah, S. (2018). Perbandingan Metode Ekstraksi Terhadap Rendemen Ekstrak Daun Rambui Laut (Sonneratia caseolaris L. Engl). Jurnal Ilmiah Manuntung, 4(1), 79–83.

License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors who publish with Sasambo Journal of Pharmacy (SJP), agree to the following terms:

Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Lisensi Creative Commons Atribusi 4.0 Internasional. This license allows authors to use all articles, data sets, graphics and appendices in data mining applications, search engines, web sites, blogs, and other platforms by providing an appropriate reference. The journal allows the author(s) to hold the copyright without restrictions and will retain publishing rights without restrictions.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in Sasambo Journal of Pharmacy
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).

Influence of extraction method on total phenolic content and antioxidant activity of Sappan Wood (Caesalpinia sappan L.) extract

Authors

DOI:

Published:

Issue:

Keywords:

Articles

Downloads

How to Cite

Abstract

References

License

Current Issue

Information

Developed By

Official Website Of

Jurnal Farmasi, Fakultas Kedokteran, Universitas Mataram

Contact Us

Official link

Share & Follow