Vol. 5 No. 2 (2024): September
Open Access
Peer Reviewed

Optimasi fase gerak dan analisis kadar natrium benzoat pada beberapa sirup rasa jeruk dengan metode kromatografi cair kinerja tinggi (kckt)

Authors

Ainil Fithri Pulungan , Zhazha Santasa

DOI:

10.29303/sjp.v5i2.372

Downloads

Received: May 10, 2024
Accepted: Sep 30, 2024
Published: Sep 30, 2024

Abstract

With the increasing growth of food and beverages in Indonesia, there has been an increase in the manufacture of soft drinks that use the preservative sodium benzoate, particularly in syrup. The concentration of sodium benzoate in beverages should be monitored, because excessive consumption can emerge various health problems. To obtain maximum analysis results, mobile phase optimization is first carried out, which aims to obtain the optimal type of eluent from several types of mobile phase, then proceed with adjusting the sodium benzoate content in the orange-flavored syrup using the HPLC method to establish whether it fits the standards in line with the BPOM Regulation No. 11 of 2019. After optimizing the mobile phase, the most optimal mobile phase is Methanol Pro HPLC: Aquabidestilata (70:30), because in this comparison the chromatogram gives the best result the peak shape is symmetrical, tailing factor is less than 2 and the retention time is less than 10 minutesBased on the findings of the third sample research, the sodium benzoate content in orange-flavored syrup meets the requirements of BPOM Regulation No. 11 of 2019, namely 600 mg/kg.

Keywords:

High performance liquid chromatography, orange syrup, preservative, sodium benzoate

References

Ali, M. S., Karim, A. E., Mohye, M. Z., Al-metwali, A. M., & Ibrahim, S. N. (2023). Simultaneous determination of Guaifenesin, codeine phosphate, phenylephrine hydrochloride, and sodium benzoate in syrup pharmaceutical form by RP-HPLC. Al-Kitab Journal for Pure Sciences, 7(2), 173–183.

Azmi, D. A., Elmatris, E., & Fitri, F. (2020). Identifikasi Kualitatif dan Kuantitatif Natrium Benzoat pada Saus Cabai yang Dijual di Beberapa Pasar di Kota Padang. Jurnal Kesehatan Andalas, 9(1S).

Hadriyati, A., & Mukhlis Sanuddin, A. F. (2020). Analisis Kadar Natrium Benzoat pada Sirup Kayu Manis dengan Metode Kromatografi Cair Kinerja Tinggi. Jurnal Kartika Kimia, 3(2), 48–52.

Maimunah, S., Nurmala, S., Sitompul, E., & Kenedy, J. (2018). Daya Tahan Simpan Sirup Jeruk Manis (Citrus sinensis (L.) osbeck) Dengan Variasi Gula. Herbal Medicine Journal, 1(1), 10–15.

Pramitha, D. A. I., Dewi, K. A. Y., & Juliadi, D. (2019). Penetapan Kadar Pengawet Natrium Benzoat Pada Sambal Kemasan Secara Spektrofotometri UV-Vis. Jurnal Ilmiah Medicamento, 5(1).

Pulungan, A. F. (2018). Penetapan Kadar Senyawa Nitrit yang Terdapat pada Daging Olahan Sosis dengan Menggunakan Metode Spektrofotmetri Uv-Vis. JIFI (Jurnal Ilmiah Farmasi Imelda), 2(1), 8–10.

Pulungan, A. F. (2019). Dampak pengawet nitrit pada daging olahan sosis terhadap kesehatan manusia. Deepublish.

Rohmah, S. A. A. (2020). Validasi Metode Penetapan Kadar Pengawet Natrium Benzoat Pada Sari Kedelai Di 3 Kecamatan Kabupaten Tulungagung Menggunakan Spektrofotometer Uv-Vis. Stikes Karya Putra Bangsa Tulungagung.

Sariwati, A. (2021). Penetapan Kadar Benzoat Pada Sampel Makanan Siap Saji dan Mie Instan Yang Beredar di Wilayah Kediri. Jurnal Pharma Bhakta, 1(2).

Saruan, S. E., Pareta, D. N., Paat, V. I., Kanter, J. W., & Tulandi, S. S. (2022). Identifikasi Kadar Natrium Benzoat Pada Saus Tomat Yang Beredar Di Pasar Beriman Kota Tomohon. Majalah INFO Sains, 3(2), 68–71.

Shaha, A., Esrafil, M., Akter, S., Bari, L., Khan, M. S. H., Alam, M. J., Dina, P. R., & Zubair, M. A. (2024). Determination of sodium benzoate in different brands of orange juices available in Bangladesh by high-performance liquid chromatography. Food Research, 8(1), 313–318.

Syenina, A. (2011). Validasi Metode Kromatografi Cair Kinerja Tinggi (KCKT) Fase Terbalik Pada Penetapan Kadar Nikotin Dalam Ekstrak Etanolik Daun Tembakau. Universitas Sanatadarma Yogyakarta.

Xu, Q. A. (2013). Ultra-High Performance Liquid Chromatography and Its Applications. John Wiley & Sons, Inc.

Zhang, Y., Liu, Y., Meng, Q., Zhou, Z., & Wu, H. (2020). A mixture of potassium sorbate and sodium benzoate improved fermentation quality of wholeâ€plant corn silage by shifting bacterial communities. Journal of Applied Microbiology, 128(5), 1312–1323.

Author Biographies

Ainil Fithri Pulungan, Universitas Muslim Nusantara Al Washliyah

Zhazha Santasa, Universitas Muslim Nusantara Al Washliyah

Downloads

Download data is not yet available.

How to Cite

Pulungan, A. F., & Santasa, Z. (2024). Optimasi fase gerak dan analisis kadar natrium benzoat pada beberapa sirup rasa jeruk dengan metode kromatografi cair kinerja tinggi (kckt). Sasambo Journal of Pharmacy, 5(2), 97–100. https://doi.org/10.29303/sjp.v5i2.372