Analisis mutu fisik, mikrobiologi, dan kandungan metabolit sekunder serbuk instan jamu kunyit asam

Abstract

The lifestyle “back to nature” is growing and in demand by the public. Herbal turmeric tamarind is a herbal product often consumed by the public to reduce pain during menstruation (dysmenorrhea). This study aimed to verify the secondary metabolites in the instant herbal turmeric tamarind powder developed by the research team using the thin-layer chromatography method.  TLC was identified by extracting instant powder of tamarind turmeric herbs using ethanol, methanol, distilled water, chloroform, ethyl acetate, and n-hexane solvents. The stationary phase used is a 60 GF ₂₅₄ silica plate and the detection of compounds by UV₂₅₄, UV₃₆₆, and chemical reagents. Identification results were analyzed descriptively by looking at the stains on the plates, calculating the Rf value of each compound, and carrying out a comparative analysis of the literature with reference standards. The results showed that the processed tamarind herbal turmeric instant powder sample of the research team positively contained alkaloids, flavonoids, tannins, steroids, and curcumin characterized by the presence of colored spots, and the resulting Rf values ​​were the same or close to those of the sample and the standard for each compound.