Skrining fitokimia ekstrak air dan ekstrak etanol 70% Propolis Trigona sp. asal Lombok Utara
Penulis
Muhammad Naufal Farras Ananta , Imasayu Nuralyza , Kurnia Solehah , Iman Surya Pratama , Siti Rahmatul AiniDOI:
10.29303/sjp.v5i1.305Diterbitkan:
2024-04-30Terbitan:
Vol 5 No 1 (2024): AprilKata Kunci:
phytochemical screening, propolis, Trigona sp., water, ethanol 70%Articles
##submission.downloads##
Cara Mengutip
Abstrak
Propolis Trigona sp. has many benefits closely related to the secondary metabolites contained therein. Previous research related to propolis screening of Trigona sp. from North Lombok has been carried out. Still, it has yet to be carried out in the maceration method (1:20 w/v) with 70% water and ethanol solvent to obtain a comparison of secondary metabolites between the two solvents. This study aims to identify the content of secondary metabolites in the water extract and 70% ethanol extract of propolis Trigona sp. from North Lombok. Propolis was macerated with water and 70% ethanol solvent. The viscous extract was identified for its physical characteristics, consistency, color, and chemical characteristics through phytochemical screening. The results of the phytochemical screening showed that the aqueous extract and 70% ethanol extract contained alkaloids, flavonoids, tannins, triterpenoids, and quinones. Both extracts showed negative saponin results.Referensi
Affandy, F., Wirasisya, D. G., & Hanifa, N. I. (2021). Skrining fitokimia pada tanaman penyembuh luka di Lombok Timur. Sasambo Journal of Pharmacy, 2(1), 1-6. DOI: https://doi.org/10.29303/sjp.v2i1.84
Ambaro, F. Y., Darusman, F., & Dewi, M. L. (2020). Prosedur Ekstraksi Maserasi Daun Bidara Arab (Ziziphus spina-christi L.) Menggunakan Pelarut Etanol dan Air. Prosiding Farmasi, 6(2), 890-893. DOI: http://dx.doi.org/10.29313/.v6i2.24050
Astarina, N. W. G., Astuti, K. W., Warditiani, N. K. 2013. Skrining fitokimia ekstrak metanol Rimpang Bangle (Zingiber purpureum Roxb.). Jurnal Farmasi Udayana, 2(4), 1-7.
Bankova, V., Trusheva, B., & Popova, M. (2021). Propolis extraction methods: A review. Journal of apicultural research, 60(5), 734-743. DOI: https://doi.org/10.1080/00218839.2021.1901426
Das, B. K., Al-Amin, M. M., Russel, S. M., Kabir, S., Bhattacherjee, R., & Hannan, J. M. A. (2014). Phytochemical screening and evaluation of analgesic activity of Oroxylum indicum. Indian journal of pharmaceutical sciences, 76(6), 571-575.
Depkes, R. I. (2000). Parameter standar umum ekstrak tumbuhan obat. Jakarta: Departemen Kesehatan Republik Indonesia, 31.
Desinta, T. (2015). Penentuan Jenis Tanin Secara Kualitatif dan Penetapan Kadar Tanin dari Kulit Buah Rambutan (Nephelium Lappaceum L.) Secara Permanganometri. CALYPTRA, 4(1), 1-10.
Ding, Q., Sheikh, A. R., Gu, X., Li, J., Xia, K., Sun, N. (2021). Chinese Propolis: Ultrasound‐assisted enhanced ethanolic extraction, volatile components analysis, antioxidant and antibacterial activity comparison. Food Science & Nutrition, 9(1), 313-330. DOI: https://doi.org/10.1002/fsn3.1997
Hasan, A. E. Z., Sunarti, D. M. T. C., Suparno, O., & Setiyono, A. (2013). Optimasi ekstraksi propolis menggunakan cara maserasi dengan pelarut etanol 70% dan pemanasan gelombang mikro serta karakterisasinya sebagai bahan antikanker payudara. Jurnal Teknologi Industri Pertanian, 23(1). 13-21.
Hikmawanti, N. P. E., Fatmawati, S., Arifin, Z., & Vindianita, V. (2021). Pengaruh variasi metode ekstraksi terhadap perolehan senyawa antioksidan pada daun katuk (Sauropus androgynus (L.) Merr). J Farm Udayana, 10(1), 1-12. DOI: https://doi.org/10.24843/JFU.2021.v10.i01.p01
Khairunnisa, K., Mardawati, E., & Putri, S. H. (2020). Karakteristik Fitokimia dan Aktivitas Antioksidan Ekstrak Propolis Lebah Trigona Sp. Jurnal Industri Pertanian, 2(1).
Marliana, S. D., Suryanti, V., & Suyono, S. (2005). Skrining fitokimia dan analisis kromatografi lapis tipis komponen kimia buah labu siam (Sechium edule Jacq. Swartz.) dalam ekstrak etanol. Biofarmasi, 3(1), 26-31.
Maya, S. W. (2015). Phytochemical screening and antipyretic effect of stem juice from kepok banana (Musa paradisiaca L) on white male rats stain wistar (Rattus norvegicus) induced with DTP-Hb. Pharmacon, 4(1). DOI: https://doi.org/10.35799/pha.4.2015.6475
Milah, N., Bintari, S. H., & Mustikaningtyas, D. (2016). Pengaruh konsentrasi antibakteri propolis terhadap pertumbuhan bakteri Streptococcus pyogenes secara in vitro. Life Science, 5(2), 95-99.
Muthmainnah, B. (2019). Skrining fitokimia senyawa metabolit sekunder dari ekstrak etanol buah delima (Punica granatum L.) dengan metode uji warna. Media Farmasi, 13(2), 36-41. DOI: https://doi.org/10.32382/mf.v13i2.880
Nagai, T., Inoue, R., Inoue, H., & Suzuki, N. (2003). Preparation and antioxidant properties of water extract of propolis. Food chemistry, 80(1), 29-33. DOI: https://doi.org/10.1016/S0308-8146(02)00231-5
Noer, S., Pratiwi, R. 2016. Uji kualitatif fitokimia Daun Ruta Angustifolia. Faktor Exacta. 9(3). 200-206. DOI: http://dx.doi.org/10.30998/faktorexacta.v9i3.879
Padmasari, P. D., Astuti, K. W., & Warditiani, N. K. (2013). Skrining fitokimia ekstrak etanol 70% rimpang bangle (Zingiber purpureum Roxb.). Jurnal Farmasi Udayana, 2(4).
Pratami, D. K., Desmiaty, Y., Simorangkir, E. M., & Faradhila, D. (2021). Standardisasi dan uji aktivitas antioksidan ekstrak bahan alam propolis untuk terapi infeksi SARS-CoV2. Jurnal Ilmu Kefarmasian Indonesia, 19(2), 272-280.
Puspitasari, L., Swastini, D. A., & Arisanti, C. I. A. (2013). Skrining fitokimia ekstrak etanol 95% kulit buah manggis (Garcinia mangostana L.). Jurnal Farmasi Udayana, 2(3), 1-4.
Putri, W. S., Warditiani, N. K., dan Larasanty, L. P. F. (2013). Skrining Fitokimia Ekstrak Etil Asetat Kulit Buah Manggis (Garcinia mangostana L). Journal Pharmacon, 09 (4), 56– 59.
Rahmayani, N., Nazaruddin, N., & Amaro, M. (2021). PENGARUH KONSENTRASI PROPOLIS TERHADAP MUTU KIMIA, MIKROBIOLOGI DAN ORGANOLEPTIK YOGHURT. Jurnal Teknologi Pangan, 15(1). DOI: https://doi.org/10.33005/jtp.v15i1.2743
Rakhmat, A. S., Hasyim, W., & Huda, M. (2021). Meningkatkan Pendapatan Masyarakat melalui Program Kemitraan Budidaya Lebah Trigona. IKRA-ITH ABDIMAS, 4(3), 230-235.
Riendriasari, S. D., & Krisnawati, K. (2017). Produksi propolis mentah (raw propolis) lebah madu trigona spp di pulau lombok. ULIN: Jurnal Hutan Tropis, 1(1). DOI: http://dx.doi.org/10.32522/ujht.v1i1.797
Rismawati, S. N., & Ismiyati, I. (2017). Pengaruh variasi pH terhadap kadar flavonoid pada ekstraksi propolis dan karakteristiknya sebagai antimikroba. Jurnal Konversi, 6(2), 89-94. DOI: https://doi.org/10.24853/konversi.6.2.89-94
Rivera-Yañez, C. R., Ruiz-Hurtado, P. A., Mendoza-Ramos, M. I., Reyes-Reali, J., García-Romo, G. S., Pozo-Molina, G., Rivera-Yañez, N. (2021). Flavonoids present in propolis in the battle against photoaging and psoriasis. Antioxidants, 10(12), 2014. https://doi.org/10.3390/antiox10122014
Sa’adah, L. (2010). Isolasi dan identifikasi senyawa tanin dari daun belimbing wuluh (Averrhoa bilimbi l.). Universitas Islam Negeri (UIN) Maulana Malik Ibrahim, Malang.
Sativa, N., & Agustin, R. (2018). Analisis uji kadar senyawa dan uji antioksidan ekstrak propolis coklat dari lebah Trigona sp. JAGROS: Jurnal Agroteknologi dan Sains (Journal of Agrotechnology Science), 2(2), 61-68.
Suhendra, S. T., & Feby Nopriandy, S. T. (2021). Lebah Trigona: Petunjuk Budidaya dan Teknis Panen Madu. Penerbit Insan Cendekia Mandiri.
Šuran, J., Cepanec, I., Mašek, T., Radić, B., Radić, S., Tlak Gajger, I., & Vlainić, J. (2021). Propolis extract and its bioactive compounds—From traditional to modern extraction technologies. Molecules, 26(10), 2930. https://doi.org/10.3390/molecules26102930
Suriawanto, N., & Setyawati, E. (2021). PENGARUH PEMBERIAN EKSTRAK PROPOLIS LEBAH TANPA SENGAT PADA PENYEMBUHAN LUKA BAKAR TIKUS PUTIH (Rattus norvegicus). Jurnal Bioteknologi & Biosains Indonesia (JBBI), 8(1), 68-76. DOI: https://doi.org/10.29122/jbbi.v8i1.4585
Susanti, N. M. P., Budiman, I. N. A., & Warditiani, N. K. (2014). Skrining fitokimia ekstrak etanol 90% daun katuk (Sauropus androgynus (L.) Merr.). Jurnal Farmasi Udayana, 3(1), 83-86.
Sylvia, D., Safitri, M., & AlHuda, Y. R. (2022). Physical Properties Test On The Formulation Of Honey Propolis (Trigona Sp) Scrub and Aloe Vera (Aloe Vera) Skin For Body Treatment. Jurnal Ilmiah Farmako Bahari Diana Sylvia, 13(2). DOI: http://dx.doi.org/10.52434/jfb.v13i2.1407
Thamrin, A. (2016). Uji Fitokimia, Toksisitas serta Antioksidan Ekstrak Propolis Pembungkus Madu Lebah Trigona Incisa dengan Metode 2, 2-diphenyl-1-picrylhidrazyl (DPPH). Jurnal Kimia Mulawarman, 14(1). 54-60.
Vifta, R. L., & Advistasari, Y. D. (2018). Skrining Fitokimia, Karakterisasi, dan Penentuan Kadar Flavonoid Total Ekstrak dan Fraksi-Fraksi Buah Parijoto (Medinilla speciosa B.). In Prosiding Seminar Nasional Unimus (Vol. 1), pp. 8-14.
Widayanti, S. M., Permana, A. W., & Kusumaningrum, H. D. (2009). Kapasitas dan kadar antioksidan ekstrak tepung kulit buah manggis (Garcinia mangostana L.) pada berbagai pelarut dengan metode maserasi. Indonesian Journal of Agricultural Postharvest Research, 6(2), 61-68. DOI: https://dx.doi.org/10.21082/jpasca.v6n2.2009.61-68
Wulansari, I. D., Admadi, B., & Mulyani, S. (2020). Pengaruh Suhu Penyimpanan terhadap Kerusakan Antioksidan Ekstrak Daun Asam (Tamarindusindica L). J. Rekayasa Dan Manaj. Agroindustri, 8(4), 544-550. DOI: https://doi.org/10.24843/JRMA.2020.v08.i04.p07
Yıldırım, H. K. (2022). Assessment of propolis treated by different extraction methods. Brazilian Archives of Biology and Technology, 65. 2-11. DOI: https://doi.org/10.1590/1678-4324-2022210251
Zahra, N. N., Muliasari, H., Andayani, Y., & Sudarma, I. M. (2021). Karakteristik fisikokimia ekstrak madu dan propolis Trigona sp. asal Lombok Utara. Jurnal Agrotek Ummat, 8(1), 7-14. DOI: https://doi.org/10.31764/jau.v8i1.3826
Lisensi
Hak Cipta (c) 2024 The Author(s)
Artikel ini berlisensi Creative Commons Attribution 4.0 International License.
Authors who publish with Sasambo Journal of Pharmacy (SJP), agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Lisensi Creative Commons Atribusi 4.0 Internasional. This license allows authors to use all articles, data sets, graphics and appendices in data mining applications, search engines, web sites, blogs, and other platforms by providing an appropriate reference. The journal allows the author(s) to hold the copyright without restrictions and will retain publishing rights without restrictions.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in Sasambo Journal of Pharmacy
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).